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Azotobacter sp is a gram-negative soil–dwelling organism with a wide variety of metabolic capabilities which includes the ability to fix atmospheric nitrogen by converting it to ammonia. In this study, 45 samples were positive out of 50 marine samples were collected in different locations of Rameshwaram marine region at the depth of 1-2m. The nitrogen fixing ability of the isolated Azotobacter sp from marine source is compared with soil Azotobacter sp and standard Azotobacter sp by pot cultivation method by assessing the growth of Green gram. Seed germination, shoot length, Root length and chlorophyll content were measured. Therefore irrespective of the environmental, marine Azotobacter sp is capable of fixing atmospheric nitrogen in a terrestrial environmental at a higher frequency rate and since it is a non-symbiont, it can be a suitable for all kind of crop plants as a biofertilizer.